Summary
Type: SPE
Application #: 305330
Catalog#: 730735 - SPE Cartridges, Chromabond, HR-XCW, 3mL, 60mg, 30/pk
Detection:
Conditions: Column conditioning: 2 ml methanol, then 2 ml water
(Do not let run the column dry!)
Sample aspiration: 1 ml sample solution is passed through the column by vacuum.
Washing: 2 ml 25 mM KH2PO4, pH 5.0, then 2 ml methanol
Drying: with vacuum
Elution: 2 ml methanol + 2 % formic acid
Evaporation: to dryness (mild nitrogen stream, slightly warming)
Reconstitution: in suitable eluent
Authors: SPE department
Source: Macherey-Nagel, Germany 2009